Anticancer effects of Rhinacanthus nasutus and Acanthus ebracteatus extracts against human cervical cancer cells

Cervical cancer is second only to breast in terms of incidence; however, it the most lethal form among Thai women due asymptomatic nature its early stages. This work aimed examine cytotoxic and antiproliferative capacities Rhinacanthus nasutus (RN) Acanthus ebracteatus (AE) extracts against human cervical cells (HeLa). Plant leaves were used for ethyl acetate extraction. The antioxidant assays, HPLC analysis, a MTT assay, clonogenic assay real-time PCR conducted. Both RN AE displayed similar DPPH scavenging activity (3.97 4.05 mg TE/g DW) ferric reducing power (4.79 4.35 Fe2+/g DW). However, was richer total phenolic content than (13.30 10.84 GAE/g DW, respectively). Rutin, catechin, chlorogenic acid, cinnamic acid found AE, whilst with much higher RN. Higher cytotoxicity 91.73% HeLa (IC50 value 62.06 µg/mL). showed effect 25.24 µg/mL) (34.35 Genes (Bcl-2, Bax, MMP-2, MMP-9, caspase-3, p21, cyclin D1) proteins (cytochrome c, caspase-3 p21) linked apoptosis migration substantially more affected by To conclude, both hold promise as anticancer herbal plants cancer; cells. offers better alternative medicine or complementary remedy standard drug treatment.